Understanding the reasons for behavioral failure: a process view of psychosocial barriers and constraints to pro-ecological behavior

WOS:000324049900008 (Nº de Acesso Web of Science)For many years now, behavior change projects and research on pro-ecological behavior seem to have encountered difficulties in answering the question: why do people fail to act? That is, what are the barriers and constraints that prevent people from acting in a pro-ecological way? In order to fill the gap, this paper aims to operationalize the concepts of barriers and constraints, based on an approach that considers the role of behavioral goals ("to achieve X"). In addition, it aims to present a preliminary approach focused on understanding the processes involved in the barriers and constraints emergence and their consequent effect on the implementation of behavioral goals into behaviors. This is done in order to allow for a better understanding of: (1) how the interaction between individual/dispositional characteristics and the characteristics of the situation in which individuals are in, may result in the inhibition/constraining of pro-ecological goals implementation into behaviors; and (2) the role of conscious and unconscious processes in this. Examples of barriers and constraints will be given, in order to make salient the need for future research to address these and for behavioral change projects to take them into consideration

Anticancer peptides : prospective innovation in cancer therapy

© Springer International Publishing Switzerland 2016Current cancer treatments require improvements in selectivity and efficacy. Surgery, radiation, and chemotherapy approaches result in patient’s suffering over time due to the development of severe side-effects that simultaneously condition adherence to therapy. Biologically active peptides, in particular antimicrobial peptides (AMPs), are versatile molecules in terms of biological activities. The cytotoxic activities of several AMPs turn this group of molecules into an amazing pool of new templates for anticancer drug development. However, several unmet challenges limit application of peptides in cancer therapy. The mechanism(s) of action of the peptides need better description and understanding, and innovative targets have to be discovered and explored, facilitating drug design and development. In this chapter, we explore the natural occurring AMPs as potential new anticancer peptides (ACPs) for cancer prevention and treatment. Their modes of action, selectivity to tumor compared to normal cells, preferential targets, and applications, but also their weaknesses, are described and discussed.info:eu-repo/semantics/publishedVersio

Does a shell matter for defence? Chemical deterrence in two cephalaspidean gastropodes with calcified shells

Opisthobranch molluscs show an evolutionary trend to reduce, internalize and lose the shell. Many of them base their defensive strategies on natural deterrent products and current evolutionary theory suggests that the acquisition of chemical defences preceded shell reduction and loss, which has characterized the evolution of this group. Here we show that basal, shelled opisthobranch molluscs are defended against sympatric predators even if their protective shell is removed. The cephalaspideans Bulla striata and Haminoea orbignyana, both with distinct shell calcification, significantly deterred feeding by sympatric crab and fish predators, both in laboratory and field assays. However, our results argue against a progressive increment of chemical defences associated with shell reduction, because the cephalaspidean with the more fully calcified shell, Bulla striata, was also the more deterrent. These findings suggest that effective chemical defences might have evolved independently from shell loss, at least in basal opisthobranchs such as cephalaspideans

Antracologia, uma nova fonte de informaçoes para a arqueologia brasileira

Charcoal analysis is the taxonomic and quantitative study of charcoal remains found in soils or archaeological deposits, based on plant anatomy. This discipline may provide both ethnoarchaeological and palaeoecological evidences. In this paper, we present its methods and possible applications, as well as a few examples. We suggest that important results may be expected from charcoal analysis applied to brazilian archaeology. (Résumé d'auteur

Lactobacillus crispatus as the etiological agent in cytolytic vaginosis

Introduction: Lactobacillus spp. dominate the vaginal niche but can also be involved in other vaginal dysbiosis, such as cytolytic vaginosis (CV), which remains poorly studied. It is characterized by a cryptic symptomatology, that often confounds the clinic. Goals: The aim of this work was to search for the etiological agent of CV, by studying the vaginal microbiome and metabolomics of women afflicted with this disease and compare it with women with other clinical diagnostic. Methods: Twenty-one vaginal washes have been collected from women attending a gynaecology consultation of a private clinic. The samples were categorized according with clinical diagnosis at the time of sampling (CV, 11; vulvovaginal candidosis, 8; Healthy, 2). The distribution of bacterial species, and their prevalence was assessed by next-generation sequencing of the 16S V4 region. In addition, total lactate D-lactic acid and L-lactic acid was quantified in all washes by a commercial kit, as well as lactate dehydrogenase (LDH) activity. Results: L. crispatus was dominant (>70%) in all CV samples. Lactate was increased in CV in comparison with other cases. The presence of D-lactic acid isomer was associated with presence of L. crispatus. LDH activity was increased in vaginal washes that tested positive for the presence of L. crispatus, however no direct association was found with CV cases. Discussion/Conclusions: The microbiome of women afflicted with CV was dominated in all cases by L. crispatus, contrarily with the results obtained for women diagnosed with other clinical symptomatology. In addition, the finding that an increase in D-lactic acid is associated with CV patients can be related to the role of L. crispatus in CV. The determination of LDH activity did not correlate exclusively with CV cases. On the other hand, D-lactic acid and total lactate quantification could be used as a valuable biomarker to diagnose this cryptic vaginal infection.info:eu-repo/semantics/publishedVersio

Infrared tracking system for immersive virtual environments

In this paper, we describe the theoretical foundations and engineering approach of an infrared-optical tracking system specially design for large scale immersive virtual environments (VE) or augmented reality (AR) settings. The system described is capable of tracking independent retro-reflective markers arranged in a 3D structure (ar-tefact) in real time (25Hz), recovering all possible 6 Degrees of Freedom (DOF). These artefacts can be ad-justed to the user’s stereo glasses to track his/her pose while immersed in the VE or AR, or can be used as a 3D input device. The hardware configuration consists in 4 shutter-synchronized cameras attached with band-pass infrared filters and the artefacts are illuminated by infrared array-emitters. The system was specially designed to fit a room with sizes of 5.7m x 2.7m x 3.4 m, which match the dimensions of the CAVE-Hollowspace of Lousal where the system will be deployed. Pilot lab results have shown a latency of 40ms in tracking the pose of two ar-tefacts with 4 infrared markers, achieving a frame-rate of 24.80 fps and showing a mean accuracy of 0.93mm/0.52º and a mean precision of 0.08mm/0.04º, respectively, in overall translation/rotation DOFs, fulfill-ing the system requirements initially defined.info:eu-repo/semantics/publishedVersio

Non-invasive hemodynamic evaluation by Doppler echocardiography

The approach for treating a hemodynamically unstable patient remains a diagnostic and therapeutic challenge. Stabilization of the patient should be rapid and effective, but there is not much room for error. This narrow window of intervention makes it necessary to use rapid and accurate hemodynamic evaluation methods. Echocardiography is the method of choice for the bedside evaluation of patients in circulatory shock. In fact, it was intensive care physicians who recognized the potential of Doppler echocardiography for the initial approach to patients in circulatory failure. An echocardiogram allows rapid anatomical and functional cardiac evaluation, which may include non-invasive hemodynamic evaluation using a Doppler study. Such an integrated study may provide data of extreme importance for understanding the mechanisms underlying the hemodynamic instability of the patient to allow the rapid institution of appropriate therapeutic measures. In the present article, we describe the most relevant echocardiographic findings using a practical approach for critical patients with hemodynamic instability.info:eu-repo/semantics/publishedVersio

International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant food

A coordinated survey for Cronobacter and related organisms in powdered infant formula, follow up formula and infant foods was undertaken by 8 laboratories in 7 countries in recognition of and in response to the data needs identified in an FAO/WHO call for data in order to develop global risk management guidance for these products. The products (domestic and imported) were purchased from the local market and were categorised according to their principle ingredients. A total of 290 products were analysed using a standardised procedure of pre-enrichment in 225 ml Buffered Peptone Water (BPW), followed by enrichment in Enterobacteriaceae Enrichment (EE) broth, plating on the chromogenic Cronobacter Druggan–Forsythe–Iversen (DFI) agar and presumptive identification with ID 32 E. Presumptive Cronobacter isolates were identified using 16S rRNA gene sequence analysis. Aerobic plate counts (APC) of the products were also determined on nutrient agar. Fourteen samples had APC > 105 cfu/g, 3 of which contained probiotic cultures. C. sakazakii was isolated from 27 products; 3/91 (3%) follow up formulas (as defined by Codex Alimentarius Commission), and 24/199 (12%) infant foods and drinks. Hence C. sakazakii was less prevalent in follow up formula than other foods given to infants over the same age range. A range of other bacteria were also isolated from follow up formulas, including Acinetobacter baumannii, Enterobacter cloacae, Klebsiella pneumoniae, Citrobacter freundii, and Serratia ficaria. There was significant variation in the reconstitution instructions for follow up formulas. These included using water at temperatures which would enable bacterial growth. Additionally, the definition of follow up formula varied between countries